Janssen Research & Development, L.L.C., a division of Johnson & Johnson's Family of Companies is recruiting for a Scientist, Protein Engineering and In Vitro Discovery located in Cambridge, MA.

At the Janssen Pharmaceutical Companies of Johnson & Johnson, we are working to create a world without disease. Transforming lives by finding new and better ways to prevent, intercept, treat and cure disease inspires us. We bring together the best minds and pursue the most promising science. We are Janssen. We collaborate with the world for the health of everyone in it. Learn more at www.janssen.com and follow us @JanssenGlobal. Janssen Research & Development, LLC is part of the Janssen Pharmaceutical Companies.

The Biologics Discovery group within the Therapeutics Discovery (TD) organization of Janssen R&D is engaged in the discovery and optimization of novel antibody and antibody based molecular entities in partnership with all therapeutic areas of Janssen. In this role you will learn about the exciting science Janssen is exploring in protein engineering that will feed into antibody and other therapeutic modalities. This position offers a phenomenal opportunity for you to engage in the discovery of next generation Biotherapeutics to address unmet medical needs as part of an outstanding team of scientists. As a member of the Protein Optimization team, you will be responsible for antibody design and optimization with a strong focus on affinity, developability and other critical attributes using structure and modeling-based design, display technologies and other emerging computational methods for protein property assessment and design. The group will also be responsible to implement high throughput screening and/or selection for the optimized antibody/protein variants to support the development of biologics such as multispecific antibodies, CAR-T/NKs, ADCs in various disease areas. Janssen provides competitive benefits and an unrivaled network of colleagues and company-sponsored activities while supporting employees in maintaining a healthy work-life balance.

Key Responsibilities:

• Design and clone antibody and protein based molecular entities
• Support the broad protein engineering efforts in the modality agnostic Therapeutics Discovery organization
• Perform and expand established assays as well as develop and optimize new assays. Responsibilities will include cloning, plasmid scale-up, plasmid QC, cell culture, reagent preparation, E. coli and mammalian cell culture, ELISA and other binding assays and flow cytometry.
• Design and perform on display-based technologies for antibody optimization, with an emphasis on phage and yeast display techniques.
• Be able to independently process data, interpret results, update the database, and present results to applicable functional groups.
• Manage laboratory operations including such as reviewing of experimental protocols and equipment manuals, writing protocols, ordering of reagents and supplies, inventory management, instrument QC, scheduling of equipment maintenance, safety inspections
• Train new hires and mentor other scientists, contractors, and co-op students

Qualifications

Education:

• MSc or PhD or equivalent in Molecular Biology, Biochemistry, Biotechnology, Biomedical Engineering, structural biology or related field is required
• A minimum of 3 years of relevant laboratory experience is required

Experience and Skills:

Required:

• Proficient knowledge of cellular/molecular biology, common biology laboratory assays such as ELISA, Luminex, and biolayer interferometry (Octet) and other assays are required
• In-depth understanding and experience of antibody engineering is required
• Innovative approach and ready to step out of the box for creating and exploring new ideas with calculated risk taking is required
• Demonstrated experience working in a cross-functional organization is required
• Strong written and oral communication skills are required

Preferred:

• Maintain accurate inventory of generated plasmids, lab notebooks, and other records and writing of technical reports, summaries, protocols, and manuscripts are preferred
• Background in managing incoming requests, conducting design and inventory registration of plasmids in a central database system is preferred
• Experience in molecular biology techniques including bi-, multi-specifics, scFv, and/or chimeric antigen receptor (CAR) construct design and cloning is preferred
• Experience in display-based technologies is preferred
• Experience with protein production using transient transfection/stable cell line generation using CHO and HEK-293 is preferred

Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.